The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. used in the SPECTRAmax PLUS. 5 mL sample or standard solution was well mixed with 0. Details. Includes: Computer Loaded with Softmax Pro 5. Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Software (SoftMax Pro ® software or comparable plate reader software). GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. SpectraMAX 340. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. 3. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. SpectraMax Multi-Mode Microplate Readers. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. 7 macrophages were obtained from ATCC (Manassas, VA). ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Unit powers. The SPECTRAmax 340PC can measure. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. 2 g/L solution; dilute 1:10 in HBSS. Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Table 1. well, and strip-well microplates. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. D2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax Multi-Mode Microplate Readers. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Samples with OD values of less than 0. The plates were incubated for 4 additional hours at 37°C. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. The growth. 5% phosphoric acid) in microplates at room temperature for 10 min. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. For use with Spectramax 340 devices which have an 8-pin mini DIN serial socket. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 2. Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). 06 ng/mL; FSH: 0. The values were corrected for nonspecific background by subtracting the OD 650. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 2. In this study we used tetanus toxoid (TT) as a well-defined antigen to. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax. Each experiment was performed in duplicate and repeated at. Briefly, cells were seeded into a. 316 grating motor driver faulty. Create custom fluorescence assays without having to purchase expensive filter sets. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. SpectraMax Injector Cartridge with SmartInject™ Technology. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). Cytotoxicity was determined by measuring the release of LDH. chen@case. The mixture changed to a red color by a reaction of LDH that was measured by a plate reader (SpectraMax 340 PC; Molecular Devices, San Jose, CA, USA) at a visible wavelength of. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The concentration of TNFα in the experimental. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Assay setup for a 384-well low-volume plate. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). These isotypes cause antigen cross-linking by multimeric binding and self. SpectraMax QuickDrop Micro-Volume Spectrophotometer. After washing, the dye was eluted with 3% HCl and the absorbance measured at 665 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Intra- and inter-assay coefficients of variation were lower than 8. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. Customer Transformation Hub. \r plates can be used in the SPECTRAmax PLUS 384. MCE has not independently confirmed the accuracy of these methods. Not all manufacturers' microplates are the same with regard to design, materials, orSpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. To. StakMax Microplate Handling Stacker. 1 mM MnCl2 and 25 mM CHES buffer, pH 9. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). edu Contact Phone: 216-368-8962. Molecular Devices SpectraMAX 340 Microplate Reader. Donor filter: 620 nm. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. It includes both a lamp and a new lamp holder. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. addresses the needs of life science researchers who quantify very small amounts of DNA, RNA, oligos, and proteins. Microplate Readers. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. At that point halfway up the maximum transmittance value, the total width (nm) of the transmitted band is. To correct for inter. Description. The values of IC 50 represent concentrations of compounds toA microplate reader (SpectraMax M5; Molecular Devices, Sunnyvale, CA) was used to measure the absorbance at 340 nm for the collected BPW (6) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer Islets December 31, 2022 Justin J. Other technologies such as imaging. 340 nm / 70 nm. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Oxidative stress induction by H 2 O 2 and intracellular ROS analysis in L-929 cells. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). more on but is otherwise untested and sold AS-IS. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. VersaMax reads 96-well and strip-well plates. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The relative cell viability was determined as the percentage ratio of the optical densities in the medium (containing the nanoprobes at each concentration) to that of the fresh control medium. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. Page: 1/6. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Triton X-100 (see Note 2). For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. SpectraMax 190 reads 96-well and strip-well plates. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. 340 nm. 304 signal level saturation. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 5. 312 gain calibration failed. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). S. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. After washing, the dye was eluted with 3% HCl, and the absorbance measured with a SpectraMax 340 microtiter plates reader at 665 nm. We also offer depot Repair Service from our base of operations in Southern California (San Diego). Product Keyword. 2. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). plates can be used in the SPECTRAmax PLUS 384. 190–850 nm <± 1. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Driessen*,MaaikeA. Subsequently, the mixture was heated at 100 °C for 10. The contents of the. 304 signal level saturation. 303 unable to cal dark current. 1 mL of acetylacetone. Molecular Devices. 0% and ± 0. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. 4) was followed according to Vanacker et al. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Glutathione reductase (GR) activity was measured, according to Smith et al. Donor: 616/10 nm. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). using a Molecular Dynamics Model SpectraMAX 340 PC microplate reader. Not all manufacturers' microplates are the same with regard to design, materials, orAbsorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). The reaction mixture consists of 100 mM potassium phosphate buffer (pH 7. (1993). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. 0. California Lightworks uses only the highest quality LEDs from Osram. The membranes were. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. Not. SpectraMax ® Plus 384 optics. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Or scan up to 384 wells at any time to determine the best assay conditions. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). 2. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. In vivo anti-malarial assay in mice. Examples: “Press carefully to engage,” “Do not press down. As discussed below, dilution of the culture was required because cell density is not proportional to A 600 when the values are above ;0. Supplier: Molecular Devices. Molecular Devices Spectramax 340PC instructions. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. The extreme flexibility and high sensitivity of the SpectraMax GEMINI EM make it appropriate for applications within the fields of bio-A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 85%. 6) and 0. 00. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. Molecular Devices SpectraMax M5e Microplate Reader. Anti-proteolytic activity. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. It measures the optical density (OD) of samples in both 96- and 384-well microplates at a selected wavelength for a single point in time (endpoint), over a specified period of time. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536 The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. 315 can't find zero order. One plate carrier adapter is provided with the instrument. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. D. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Incubation with TMB substrate was done for 18 min. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. Amino Acid Analysis. The Catalina. ELISA plates were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Title: SPECTRAmax 340 Microplate Reader manual Author: Research Facilities Keywords: spectramax, 340, microplate reader, manual, Created Date: 12/13/2005 9:07:53 AMSpectraMax Multi-Mode Microplate Readers. 2 software was used to measure and calculate of the bioactivity activity studies. Calculating % of total mediator release in RBL assay. Also for: Spectramax 190, Spectramax 340pc384, Versamax. Details. The absorbances were being measured at 515 nm. Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 5. 94 g calcium chloride dihydrate (both. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. This light offers spectrum variability, wireless control, and photosensors to automatically balance output with natural light. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). The microtiter plate was then incubated in the dark for 2 h at 37°C. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. They are for reference only. Absorbance was recorded at 540 nm using SpectraMAX 340 reader. Number of flashes. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. DNA extraction from blood meal ELISA homogenate. Money Saved: $340. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. The Gemini EM Reader with dual monochromators allows researchers to try new and novel dyes without having toThe absorbance was determined to be 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. , Sunnyvale, CA). 5mM), and changes in OD 405 nm. SpectraMax Multi-Mode Microplate Readers. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). These lab workhorses are used in drug discovery, research, bioassay validation, and. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). Since the formation of NADPH is stoichiometrically related to the oxidation of glucose-6-phosphate, measurements for glucose + fructose can be determined by the absorbance of NADPH at 340 nm. TDP-43 stress granular cells were treated with 70% ice-cold. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Quick View. Donor filter: 616/10 nm. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Laser Scanning Confocal Microscopy The fluorescent signals were obtained using a Zeiss LSM 510 laser scanning confocal microscope. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. 0 1. Donor: 620 nm. The cell concentration was measured by a spectrometer (SpectraMax 340, Molecular Devices, USA) with an optical density at 600 nm (OD 600). The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. The chamber contains. Integration delay. Plates were washed with 1× KP solution and blocked with. One plate carrier adapter is provided with the instrument. Data were expressed as the mean percent viable cell vs. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. mums. Column chromatography was performed on silica gel (Kieselgel 60, 70–230 mesh, Merck). 2. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Read one sample or up to 384. SpectraMAX 340 plate reader (Molecular Devices). 0, 20 mmol L –1cal-cium chloride). SpectraMax Multi-Mode Microplate Readers. , Sunnyvale, CA, USA) at 595 nm. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Streamline GLP/GMP compliance. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. Description 1. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 1 mL of culture media with 10% FBS. Buffer (100 mmol L –1acetate buffer, pH 5. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. No power cord or accessories included. Immunofluorescence and Aggregation Analysis. 303 unable to cal dark current. SoftMax Pro. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. Data System. The drawer will open and it may close, if is doesn't, close it using the DRAWER button found on the Spectramax control panel (pictured in purple on. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Molecular Device M2e. Immunofluorescence and Aggregation Analysis. ). Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Wavelength Selection. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Values were determined according to a standard curve (log/log curve-fit). Acceptor: 665/10 nm. 6) and 40 μL acetylacetone, then the mixture was heated at 100 °C for 10 min to. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. SpectraMax Multi-Mode Microplate Readers. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Molecular Device M5e. Binding affinity for denosumab. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Detection limits. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. , Sunnyvale CA). #N207) LAL Reagent Water (Lonza cat. The. used. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. Stat Fax 3200 Microplate Reader. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The concentration of NO in the cells was determined by measuring the amount of nitrite, a relatively stable oxidation product of. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Spectrophotometer (SpectraMax 340). TGFα release was calculated as described in the Results. ac. The cell survival. Warranty. Novak, Nathan J. Operators can conveniently select up to 6 wavelengths at a time for. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). TDP-43 stress granular cells were treated with 70% ice-cold. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. Please see photos. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). 2 were considered negative for IgM antibody.